In vivo selection of MGMT(P140K) lentivirus–transduced human NOD/SCID repopulating cells without pretransplant irradiation conditioning
J. Clin. Invest. Steven P. Zielske, et al. 112:1561 doi:10.1172/JCI17922 [Go to this article.]

Figure 4
Analysis for the presence of MGMT(P140K) in CFUs. (a) CFU DNA from IR/untreated control mice or twice-treated mice was analyzed for the presence of MGMT(P140K) by PCR. Minus signs represent negative PCR control (water or blank methylcellulose), and plus signs represent positive PCR control (MGMT[P140K]–transduced mass culture); remaining lanes show representative CFUs as indicated. (b) Quantitation of MGMT(P140K)⁺ CFUs obtained from in vitro and pretransplant culture (n = 8 independent transductions), IR/untreated control mice (n = 2 mice), and mice treated twice with BG/BCNU (n = 4 mice). BG/BCNU treatment resulted in selection for lentivirus-transduced CFCs.