In vivo selection of MGMT(P140K) lentivirus–transduced human NOD/SCID repopulating cells without pretransplant irradiation conditioning
J. Clin. Invest. Steven P. Zielske, et al. 112:1561 doi:10.1172/JCI17922 [Go to this article.]

Figure 2
Effect of BG/BCNU and IR treatment on NOD/SCID bone marrow. Bone marrow aspirates were taken from two mice, each of which were treated 1 day prior either with 30 and 10 mg/kg BG and BCNU, respectively, or with 250 cGy IR. (a) The number of nucleated cells recovered from femurs was determined for each mouse. Femurs from IR-treated mice contained fewer cells than femurs from BG/BCNU–treated mice. (b) Following treatment, 3 × 10⁵ nucleated bone marrow cells from each mouse were plated in 4 ml methylcellulose, and CFUs were counted after 10 days. Values are percent of untreated. The number of CFCs in IR-treated mice was substantially reduced compared with that in untreated or BG/BCNU–treated mice.