Role for integrin-linked kinase in mediating tubular epithelial to mesenchymal transition and renal interstitial fibrogenesis
J. Clin. Invest. Yingjian Li, et al. 112:503 doi:10.1172/JCI17913 [Go to this article.]

Figure 1
TGF-β1 induces ILK expression in renal tubular epithelial cells. (a–d) Western blot analyses show that TGF-β1 induced ILK protein expression in a time- and dose-dependent manner. HKC cells were incubated with either the same concentration of TGF-β1 (2 ng/ml) for various periods of time (a and c) or with increasing amounts of TGF-β1 for 24 hours (b and d). Cell lysates were immunoblotted with Ab’s against ILK and actin, respectively. (c and d) Graphic presentation of relative ILK abundance (fold induction) normalized to actin. (e and f) Immunofluorescence staining shows the localization of ILK in control (e) or TGF-β1–treated HKC cells (f). Arrowheads indicate positive ILK staining. Scale bar, 5 μm.