Diabetes and diabetes-associated lipid abnormalities have distinct effects on initiation and progression of atherosclerotic lesions
J. Clin. Invest. Catherine B. Renard, et al. 114:659 doi:10.1172/JCI17867 [Go to this article.]

Figure 6
Diabetes caused advanced lesions in mice fed cholesterol-rich diets. Diabetic LDLR^–/–;GP mice fed the 0.12% cholesterol diet (A, B, and E), or 0.5% cholesterol diet (C), and nondiabetic LDLR^–/–;GP littermates fed the 0.12% cholesterol diet (F) or the 0.5% cholesterol diet (D) were perfusion fixed after 12 weeks on diet, as described in Figure 4. The BCA was dissected, paraffin embedded, and serial sectioned until maximal lesion size was identified. Sections were stained using a Movat’s pentachrome procedure (A–D). Black represents nuclei and elastin, yellow represents collagen and reticular fibers, blue represents glycosaminoglycans, red represents muscle, and intense red represents fibrinoid and fibrin (hemorrhage). Some sections were used to detect AGEs (E and F). Representative sections are shown. Note the intralesional hemorrhage (marked by arrows) in A and C. In B, erythrocytes in the lesion are indicated by open arrows. Scale bars: 100 μm (A, C–F); 20 μm (B). (G) A graphical representation of frequency of hemorrhage in lesions of similar size from diabetic and nondiabetic mice.