Diabetes and diabetes-associated lipid abnormalities have distinct effects on initiation and progression of atherosclerotic lesions
J. Clin. Invest. Catherine B. Renard, et al. 114:659 doi:10.1172/JCI17867 [Go to this article.]

Figure 5
Diabetes caused lesion initiation in mice fed a cholesterol-free diet. Diabetic LDLR^–/–;GP mice fed the 0% cholesterol diet (A–F, H–I) and nondiabetic LDLR^–/–;GP littermates fed the 0% cholesterol diet (G and J) were perfusion fixed after 12 weeks on diet, as described in Figure 4. The BCA was dissected, paraffin embedded, and serial sectioned until maximal lesion size was identified. Sections were stained using a Movat’s pentachrome procedure (A–C, E, and G). Black represents nuclei and elastin, yellow represents collagen and reticular fibers, blue represents glycosaminoglycans, red represents muscle, and intense red represents fibrinoid and fibrin (hemorrhage). Some sections were used to detect macrophages (D and F), by using a rat monoclonal Mac-2 antibody, and others were used to detect AGEs (H and J) or used as negative controls (I). Representative sections are shown. Scale bars: 100 μm.