IL-7 enhances peripheral T cell reconstitution after allogeneic hematopoietic stem cell transplantation
J. Clin. Invest. Önder Alpdogan, et al. 112:1095 doi:10.1172/JCI17865 [Go to this article.]

Figure 5
De novo–generated T cells undergo homeostatic expansion in the recipient of an allogeneic BMT. (a and b) CFSE-labeled thymocytes (Thy) (a) and splenocytes (Spl) (b) (10⁷ each) from syngeneic donors (Syn) were transferred into lethally irradiated CBA/J recipients. On day 5, splenocytes from the recipients were harvested and stained with anti-CD4 and -CD8 antibodies for flow cytometric analysis. (c–e) Lethally irradiated CBA/J mice were transplanted with B10.BR TCD-BM and sacrificed on day 28 after allogeneic BMT. Thymocytes (c) and splenic T cells (d) were harvested, pooled, and labeled with CFSE, and 10⁷ cells were infused into irradiated CBA/J mice. On day 5, splenocytes from the recipients were harvested and stained with anti-CD4 and -CD8 antibodies for flow cytometric analysis. Infusion with CFSE-labeled B10.BR splenic T cells was performed as the allogeneic control (e). CBA, CBA/J.