IL-7 enhances peripheral T cell reconstitution after allogeneic hematopoietic stem cell transplantation
J. Clin. Invest. Önder Alpdogan, et al. 112:1095 doi:10.1172/JCI17865 [Go to this article.]

Figure 4
Most de novo–generated T cells after allogeneic BMT with purified hematopoietic stem cells have a memory/activated phenotype. Lethally irradiated (1,300 cGy) CBA/J mice were transplanted with either B10.BR TCD-BM (allogeneic BMT) or lineage-negative SCA-1⁺c-kit⁺ B10.BR BM cells (2 × 10⁴). IL-7 (10 μg/day) or PBS (control) was administered by intraperitoneal injection from day 21 to day 28 after allogeneic BMT, and from day 28 to day 35 after allogeneic SCT. Animals were sacrificed and harvested on day 28 (BMT) and day 35 (SCT). Absolute numbers of specific donor-derived cell populations were calculated from total cell counts and percentages of T cells by flow cytometric analysis. Naive CD4⁺ T cells were defined as CD44^lowCD62L^high, activated CD4⁺ T cells as CD44^highCD62L^high, memory CD4⁺ T cells as CD44^highCD62L^low, naive CD8⁺ T cells as CD44^low, and memory/activated CD8⁺ T cells as CD44^high. Values represent the mean cell number ± SEM (SCT: n = 3; BMT: n = 16).