IL-7 enhances peripheral T cell reconstitution after allogeneic hematopoietic stem cell transplantation
J. Clin. Invest. Önder Alpdogan, et al. 112:1095 doi:10.1172/JCI17865 [Go to this article.]

Figure 1
T cell and B cell reconstitution after allogeneic BMT. (a and b) Lethally irradiated (1,300 cGy) CBA/J recipients were transplanted with B10.BR TCD-BM (5 × 10⁶) with or without B10.BR T cells (0.5 × 10⁶) to induce GVHD. At different timepoints after BMT, animals were sacrificed and absolute numbers of donor-derived cell populations in the spleen were calculated from total cell counts and multicolor flow cytometric analyses of T cells with anti-CD3, -CD4, and -CD8 antibodies (a), and B cells with anti-B220 antibody (b). Donor or host origin was determined with anti-Ly9.1, which is present on host leukocytes. Allo, allogeneic. (c) The dot plots demonstrate how CD44^high and CD44^low T cells can be distinguished by flow cytometric analysis in the spleen of a normal mouse and that CD44^low populations represent naive CD4⁺ T cells, which express CD62L, or naive CD8⁺ T cells, which do not express CD122. CBA/J mice were transplanted as described in Figure 1a without the addition of T cells, and splenocytes were analyzed on day 28. Naive CD4 cells were defined as CD4⁺CD44^low, memory/activated (MEM/ACT) CD4 cells were defined as CD4⁺CD44^high, naive CD8 cells were defined as CD8⁺CD44^low, and memory/activated CD8 cells were defined as CD8⁺CD44^high. (d) CBA/J mice were transplanted as described in Figure 1a without the addition of T cells and received 10 μg/day IL-7 or PBS (control) on days 21–27 by intraperitoneal injection. Splenocytes were analyzed at different timepoints after transplant. Values represent the mean cell number ± SEM (n = 6–20). *P < 0.05.