Mosaic analysis of insulin receptor function
J. Clin. Invest. Tadahiro Kitamura, et al. 113:209 doi:10.1172/JCI17810 [
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Figure 4Lipoatrophy and analysis of BAT gene expression. (
a) Representative histologic appearance of H&E-stained sections from dermal WAT, peri-epididymal WAT, and BAT. For simplicity, only Δ98 mice are shown, but the data are identical in Δ80 mice. (
b–
d) EM analysis of peri-epididymal WAT precursors in Δ98 mice. The magnifications shown are (
b) ×1,900, (
c) ×5,000, and (
d) ×46,000. Intramitochondrial inclusions of electron-dense material are shown in
b and
d. The mitochondria are enlarged and show poorly organized cristae. (
e and
f) EM analysis of BAT precursors in Δ98 mice. Numerous pre-adipocytes adjacent to small capillaries can be seen. Mitochondria are indicated by arrows. The magnifications shown are (
e) ×1,900 and (
f) ×5,000. P, pre-adipocytes; C, capillaries; L, lipid droplet. (
g) Analysis of BAT gene expression. We isolated mRNA from 3-week-old mice and performed real-time RT-PCR with primers encoding the genes indicated at the top of each panel. The data represent means ± SEM of three independent measurements (
n = 10 for each genotype). We used amplification of β-actin to normalize gene expression data. Asterisks indicate a statistically significant difference (*
P < 0.05 and **
P < 0.01 by ANOVA) between genotypes. To measure the levels of immunoreactive Pgc1α, we performed Western blotting with anti-Pgc1α antiserum. We show a representative autoradiogram and a loading control with anti-dynamin antiserum below the Pgc1α real-time PCR graph.
