Mosaic analysis of insulin receptor function
J. Clin. Invest. Tadahiro Kitamura, et al. 113:209 doi:10.1172/JCI17810 [Go to this article.]

Figure 2
In vivo generation of PIP3 in response to insulin. We injected insulin via the inferior vena cava of anesthetized mice and after 3 minutes perfused the liver with paraformaldehyde. We then processed the livers for histochemistry with anti-PIP3 antiserum as described. We applied nuclear counterstain to count cell number. We analyzed multiple sections from three mice for each genotype. A representative experiment is shown.