Mosaic analysis of insulin receptor function
J. Clin. Invest. Tadahiro Kitamura, et al. 113:209 doi:10.1172/JCI17810 [Go to this article.]

Figure 1
Evaluation of Insr mosaicism. (a) RT-PCR analysis. We isolated mRNA from liver of individual mosaic (lanes 1–6) and WT (lane 7) mice. Because of the deletion of Insr exon 4, the length of the PCR product generated from the Δ^loxP allele is smaller (350 bp) than the WT allele (500 bp) (upper panel). (b) Protein levels of Insr and Igf1r were examined by Western blotting as indicated in Methods. The first and second panels from the top show different exposures of the same autoradiogram to better visualize Insr expression in mice with greater degrees of mosaicism. The third panel from the top shows samples from the same set of mice analyzed with anti-Igf1r antiserum to normalize protein levels. (c) Expression level of Insr in various tissues in Δ80 and Δ98 mice by Western blotting. We removed brain, liver, skeletal muscle, and BAT and determined protein levels of Insr and Igf1r by Western blotting as indicated in Methods.