Antibody blockade of the Cripto CFC domain suppresses tumor cell growth in vivo
J. Clin. Invest. Heather B. Adkins, et al. 112:575 doi:10.1172/JCI17788 [Go to this article.]

Figure 3
Overexpression of Cripto blocks Act B growth suppression of breast epithelial cells. (a) T47D or T47D-hCr cells were grown in media alone (white bars) or media plus 25 ng/ml Act A (black bars) or Act B (gray bars). Proliferation was measured by an MTT assay after 8 days. n = 4; the experiment was repeated at least twice. (b) Lysates of T47D and T47D-hCr cells stimulated with 25 ng/ml of Act A (left panel) or Act B (right panel) for 0, 10, 30, and 60 minutes were immunoblotted with anti–phospho-Smad2 polyclonal antibody. B, blank sample of T47D untreated cells. The arrow points to the phospho-Smad2 band. (c) T47D and T47D-hCr cells were stimulated with 0, 5, 25, or 50 ng/ml Act A, Act B, or EGF for 1 hour and then lysed and probed for p44/42 MAPK.