Cathepsin B inactivation attenuates hepatic injury and fibrosis during cholestasis
J. Clin. Invest. Ali Canbay, et al. 112:152 doi:10.1172/JCI17740 [
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Figure 5Hepatic fibrosis is increased in
Ctsb+/+ BDL compared with
Ctsb–/– BDL mice. (
a) Two weeks after the surgical procedure, liver tissue was obtained from BDL and sham-operated
Ctsb+/+,
Ctsb–/–, and R-3032–treated
Ctsb+/+ BDL mice, and collagen fibers were stained with sirius red as described in Methods. (
b) The surface area stained with sirius red was quantitated using digital image analysis. Sirius red staining was quantitatively greater in
Ctsb+/+ BDL than in
Ctsb–/– and R-3032–treated
Ctsb+/+ BDL mice (
P < 0.001,
n = 4 for each group). Only minimal sirius red staining was observed in sham-operated mice from the three groups of animals. (Original magnification ×20.)
