Critical role of galectin-3 in phagocytosis by macrophages
J. Clin. Invest. Hideki Sano, et al. 112:389 doi:10.1172/JCI17592 [Go to this article.]

Figure 5
Localization of galectin-3 in actin-rich phagocytic cups and phagosomes. Phagocytosis of srbc’s by wild-type and gal3^–/– macrophages was performed as described in Figure 1. After the indicated time periods, unbound srbc’s were removed and the cells were fixed, permeabilized, and treated with goat anti–galectin-3 Ab, followed by FITC-conjugated mouse anti-goat IgG secondary Ab and rhodamine-phalloidin to stain galectin-3 and actin. (a) Distribution of galectin-3 (upper panels; green) and F-actin (middle panels; red) inside wild-type macrophages. An overlay of these images demonstrates colocalization of F-actin and galectin-3 (lower panels; yellow). Panels on the left show wild-type macrophages before stimulation and those on the right show 1 minute after stimulation with opsonized srbc’s. (b) Overlay of galectin-3 and F-actin in wild-type macrophages after 10 and 15 minutes of stimulation.