Critical role of galectin-3 in phagocytosis by macrophages
J. Clin. Invest. Hideki Sano, et al. 112:389 doi:10.1172/JCI17592 [
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Figure 1Delayed phagocytosis of IgG-opsonized erythrocytes by
gal3–/– macrophages in vitro. (
a) After opsonized srbc’s were added to cultured BMMΦ at 4°C, synchronized phagocytosis was initiated by raising the temperature of the cultures to 37°C. After the indicated time periods, unbound srbc’s were removed and the cells were fixed for phase-contrast microscopy. (
b) Phagocytic function was quantified by counting internalized srbc’s in more than 300 macrophages and the phagocytic index was calculated. Each data point represents the mean ± SD from five experiments. (
c) Unsynchronized phagocytosis assays in BMMΦ were performed at 37°C for 20 minutes. Data are presented as mean ± SD from five high power fields and are representative of two experiments.
P < 0.01 by Mann-Whitney
U analysis between the genotypes. (
d) Phagocytosis assays were performed as in
a after staining of rbc’s with FITC-albumin at the time of opsonization. After 30 minutes, cells were harvested, washed, and subjected to flow cytometric analysis. Results shown are representative of six experiments.
