Prevention of gastrointestinal tumors based on adenomatous polyposis coli gene mutation by dendritic cell vaccine
J. Clin. Invest. Toshio Iinuma, et al. 113:1307 doi:10.1172/JCI17323 [Go to this article.]

Figure 1
FACS analysis and fluorescence microscopy of DCs and tumor cells simply mixed or treated with PEG. DCs stained with FITC-conjugated anti-CD80 and tumor T cells stained with the red-fluorescent dye PKH26GL were mixed, treated with PEG or not, and incubated overnight as described in the text. A mixture of DCs and tumor cells that had not been treated with PEG served as control. After overnight incubation, the cells were collected, analyzed by FACS, and examined under a fluorescence microscope. FACS analysis results: (A) DCs stained with FITC-conjugated anti-CD80; (B) tumor cells stained with PKH26GL; (C) mixture of DCs and tumor cells; (D) PEG-treated DCs and tumor cells. Fluorescence microscopy: (E) DCs stained with FITC-conjugated anti-CD80; (F) tumor cells stained with PKH26GL; (G) mixture of DCs and tumor cells; (H) PEG-treated DCs and tumor cells. Six independent experiments were performed with similar results. A typical experiment is shown. FACS analysis of DCs and DC/Ts: DCs (blue solid line) and DC/T (red solid line) were stained with FITC-conjugated mAb’s against (I) H-2Kb (J) I-Ab (K) CD80, and (L) CD86. Cells stained with FITC-conjugated isotype-matched control antibody are indicated by the dotted lines (green, DCs; orange, DC/Ts).