Injection of genetically engineered fibroblasts corrects regenerated human epidermolysis bullosa skin tissue
J. Clin. Invest. Susana Ortiz-Urda, et al. 111:251 doi:10.1172/JCI17193 [Go to this article.]

Figure 1
Type VII collagen expression in primary skin cells. (a) Immunoblots of extracts from primary RDEB (EB) and normal (NL) patient skin fibroblasts and keratinocytes (KCs). The first lane represents RDEB⁺ fibroblasts engineered to overexpress type VII collagen. pCOL7A1 is a CMV-driven expression plasmid for type VII collagen (3) stably transfected using the φC31 integrase. Optical densitometric quantitation of type VII collagen protein levels are noted below each sample lane, normalized to BRG1 (25), a constitutively expressed control for extract loading, quality, and transfer; normal fibroblasts are assigned a relative value of 1.0. UD, undetectable. (b) Cellular expression of type VII protein expression (green) in engineered (RDEB⁺), normal (NL), and uncorrected (RDEB^–) fibroblasts. Counterstaining with Hoechst 33342 marks all cellular nuclei (blue).