The voltage-gated Kv1.3 K⁺ channel in effector memory T cells as new target for MS
J. Clin. Invest. Heike Wulff, et al. 111:1703 doi:10.1172/JCI16921 [Go to this article.]

Figure 1
Myelin antigen–activated T cells from MS patients express the distinctive Kv1.3^highIKCa1^lowphenotype. (a) Kv1.3 and IKCa1 currents in TCLs from MS patients and controls measured 48 hours after activation with MBP. The numbers 1, 2, and 3 in the Kv1.3 traces represent traces after the first, second, and third depolarizing 200-ms pulse; the pulse interval is 1 second. (b) Kv1.3 channel number/cell in myelin antigen–activated TCLs (left) or mitogen-activated T cells (right) from MS patients or controls. Each data point constitutes the mean ± SEM of 20–30 cells from two to seven independent TCLs. Myelin antigens used were MBP (filled and open squares), MOG (filled and open triangles), and PLP (filled diamonds). Filled symbols, MSpatients; open symbols, control subjects. Mitogens used were soluble anti-CD3 mAb (50 ng/ml) and PMA (10 nM) plus ionomycin (175 nM) (data for both mitogens are grouped together). Filled circles, MS patients; open circles, controls. (c) Kv1.3 channel number/cell in MS patient T cells activated for 48 hours with the control antigens GAD65, insulin peptide, and ovalbumin. Due to the paucity of cells in these TCLs, IKCa1 expression was measured in only two to three cells per TCL.