Surfactant proteins A and D inhibit the growth of Gram-negative bacteria by increasing membrane permeability
J. Clin. Invest. Huixing Wu, et al. 111:1589 doi:10.1172/JCI16889 [
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Figure 4Specificity of collectin-mediated growth inhibition of
E. coli K12. (
a) The purity of collectins used in the study, including the EDTA eluate of the surfactant pellet (lane 1), fast protein liquid chromatography fractions 8 (lane 2) and 12 (lane 3), rSP-A (lane 4), and rSP-D (lane 5), was assessed by silver staining after 8–16% SDS-PAGE electrophoresis. (
b)
3H-uridine incorporation by
E. coli K12 was assessed alone and in the presence of 2 mM EDTA; in the presence of hSP-A (100 μg/ml) or rSP-D (10 μg/ml) at pH 5.0, 6.0, and 7.4; in the presence of boiled hSP-A (100 μg/ml) or rSP-D (10 μg/ml); and in the presence of the ultrafiltrate (molecular weight cutoff of 10,000) of hSP-A (100 μg/ml) or rSP-D (10 μg/ml) before boiling (room temperature; RT) and after boiling (100°C). Data are mean ± SEM;
n = 3; *
P < 0.01.
