Salicylic acid attenuates virulence in endovascular infections by targeting global regulatory pathways in Staphylococcus aureus
J. Clin. Invest. Leon Iri Kupferwasser, et al. 112:222 doi:10.1172/JCI16876 [Go to this article.]

Figure 4
Fluorescence analysis of aspirin-induced effects on sarA P1:gfp_uvr constructs in vivo. Vegetative lesions were removed from rabbits with infective endocarditis 24 hours after they received no treatment (controls) or a single intravenous dose of 8 mg/kg aspirin, and fluorescence microscopy was conducted on sarA P1:gfp_uvr constructs within the lesions. In vegetations from control rabbits, bright green fluorescence (a) can be clearly detected as originating from the respective cells shown in the adjacent section stained with Giemsa (b). In comparison, the fluorescence intensities of individual bacterial cells found in Giemsa-stained vegetation sections of aspirin-treated rabbits (d) show a marked reduction in fluorescence (c, arrowheads), even at the highest immunofluorescent microscopy gain (magnification ×350).