Chemokine receptor mutant CX3CR1-M280 has impaired adhesive function and correlates with protection from cardiovascular disease in humans
J. Clin. Invest. David H. McDermott, et al. 111:1241 doi:10.1172/JCI16790 [Go to this article.]

Figure 3
Calcium mobilization by CX3CR1-M280 is defective. Data represent the mean ± SEM of one representative experiment (a) and three independent experiments (b). The cell lines used were the same as those analyzed in Figure 1. (a) Kinetics. Shown are real-time fura-2 AM fluorescence traces of untransfected HEK 293 cells or HEK 293 cells expressing the recombinant receptor indicated by the key at upper right in response to 1 μM soluble FKN added at 19 seconds. (b) Concentration dependence. Shown is the maximal fura-2 AM fluorescence change of HEK 293 cells stably transfected with the indicated construct in response to the indicated concentration of FKN. P values at the top of each set of bars compare results for CX3CR1-M280 and CX3CR1-WT by two-tailed Student t test.