Chemokine receptor mutant CX3CR1-M280 has impaired adhesive function and correlates with protection from cardiovascular disease in humans
J. Clin. Invest. David H. McDermott, et al. 111:1241 doi:10.1172/JCI16790 [Go to this article.]

Figure 2
CX3CR1-M280 has defective adhesion activity. (a) Receptor expression in transfected K562 cell lines. The name of the transfected receptor is at the top of each FACS plot. Thick lines: CX3CR1-specific mAb 2A9. Thin lines: isotype antibody control. Data are representative of three independent experiments. (b) Flow-based adhesion assay. K562 cells were perfused over monolayers of untransfected (shown as 926) and FKN-transfected (shown as 926-FKN) human endothelial cells at room temperature (RT) or 37°C and allowed to capture at a shear stress of 0.25 dynes/cm², then washed at progressively higher shear stresses up to 10 dynes/cm². The numbers of cells remaining firmly bound to 926 cell monolayers at 10 dynes/cm² are shown. Data represent the mean ± SD of results from three separate experiments at 37°C and four separate experiments at room temperature.