Mutations in factor H reduce binding affinity to C3b and heparin and surface attachment to endothelial cells in hemolytic uremic syndrome
J. Clin. Invest. Tamara Manuelian, et al. 111:1181 doi:10.1172/JCI16651 [Go to this article.]

Figure 3
Recombinant expression of wild-type factor H SCRs 8–20 and HUS mutations of factor H. (a) Culture supernatants of insect cells infected with the recombinant viruses were harvested 5 days after infection, separated by SDS-PAGE, and used for Western blot analysis. (b) Purified recombinant proteins representing the wild-type factor H protein (SCRs 8–20) or the proteins with the indicated mutations were separated by SDS-PAGE and detected by silver staining. The mobility of the marker is indicated on the left.