FGF-2 regulates neurogenesis and degeneration in the dentate gyrus after traumatic brain injury in mice
J. Clin. Invest. Shinichi Yoshimura, et al. 112:1202 doi:10.1172/JCI16618 [Go to this article.]

Figure 1
BrdU-positive cells in the SGZ of DG after CCI in FGF-2^+/+ and FGF-2^–/– mice. (a) To label dividing cells, BrdU was injected intraperitoneally at 6, 7, and 8 days after CCI or sham operation, and mice were killed on day 9. BrdU labeling was detected by immunohistochemistry (see Methods). Few BrdU-labeled cells were present within the SGZ of the ipsilateral DG in the sham-operated FGF-2^+/+ and FGF-2^–/– mice. After CCI, an increase in the number of positive cells was detected, but the increase was less in FGF-2^–/– mice. Tissue section coordinate, bregma –1.9 mm. Scale bar: 100 μm. (b) Quantification of BrdU-positive cells in DG after CCI in FGF-2^+/+ and FGF-2^–/– mice. BrdU was injected at 6, 7, and 8 days, or 13, 14, and 15 days after CCI, and animals killed on day 9 or 16, respectively. Sham-operated mice were injected with BrdU on days 6, 7, and 8, and killed on day 9. Cell proliferation was assessed by immunohistochemical detection of incorporated BrdU, and the positive cells were counted at four different levels of DG using stereologic methods. Data are expressed as the total number of labeled cells within SGZ (see Methods). After TBI, the number of BrdU-positive cells increased at 9 and 16 days in FGF-2^+/+ mice (black bars), whereas the increase in FGF-2^–/– (white bars) was present only at 9 days after injury. ^†P < 0.05 compared with sham-operated mice; *P < 0.05, FGF-2^+/+ vs. FGF-2^–/–. n = 4–6 per group.