Increased islet apoptosis in Pdx1^+/– mice
J. Clin. Invest. James D. Johnson, et al. 111:1147 doi:10.1172/JCI16537 [Go to this article.]

Figure 6
RT-PCR analysis of gene expression. (a) The densitometric quantification of four apoptosis-related genes — Bcl_XL, caspase-3 (Casp), BAX, and Bcl-2 — relative to GAPDH in cultured islets (cultured as in Figure 5) is quantified by densitometry (above). Data are pooled from four gels (representative example shown below). White bars over gel images denote Pdx1^+/+ islets, while black bars denote Pdx1^+/– islets. Each RT-PCR sample was pooled from the cultured islets of three mice. (b–d) Relative abundance of PDX1, insulin-1 (Ins-1), insulin-2 (Ins-2), and glucagon (Gluca) mRNA are quantified from the same samples. Single asterisks denote significant differences between Pdx1^+/– and Pdx1^+/+ islets. Double asterisks denote significant differences between different treatments to the same type of islet. Insulin content per islet protein was also not reduced in Pdx1^+/– islets (not shown).