Increased islet apoptosis in Pdx1^+/– mice
J. Clin. Invest. James D. Johnson, et al. 111:1147 doi:10.1172/JCI16537 [Go to this article.]

Figure 2
Evoked insulin release from populations of size-matched isolated islets is not altered in Pdx1^+/– mice. (a) Perifused islets were exposed to 20 mM glucose (black bar) and 20 mM KCl (white bar) in the same experiments. Islets from three mice of each genotype were compared. Thirty to fifty islets/column were used. Insulin secretion is normalized to pretreatment values to allow pooling of columns with different basal insulin release. Basal insulin release was approximately 1.5 μU/ml/h. (b) The response of Pdx1^+/+ and Pdx1^+/– islets to a ramp increase to 26 mM glucose is shown (n = 4). In the presence of 26 mM glucose, islets were challenged with 20 mM arginine (right). Note the different scale. (c) Groups of five physically similar islets from Pdx1^+/– mice or littermate controls were incubated for 1 hour in 2 mM glucose (G) (n = 33), 5 mM glucose (n = 12), 20 mM glucose (n = 33), or 2mM glucose plus either 20 mM KCl (n = 23), 5 μM forskolin (n = 12), 250 μM carbachol (n = 6), or 30 μM mastoparan (n = 15). Unless otherwise indicated, 2 mM glucose was used. forsk, forskolin; Cch, carbachol; masto, mastoparan.