Prolactin modulates the naive B cell repertoire
J. Clin. Invest. Elena Peeva, et al. 111:275 doi:10.1172/JCI16530 [
Go to this article.]
Figure 4Anti-DNA B cells in CD4 knockout R4A-γ2b transgenic BALB/c mice. Splenocytes were isolated from seven murine prolactin–treated and seven placebo-treated CD4 knockout R4A-γ2b BALB/c mice after 4 weeks of treatment and were evaluated for γ2b expression and the number of spontaneously activated DNA-reactive B cells. (
a) Prolactin- and placebo-treated mice displayed the same difference in the percentage of B cells expressing γ2b. The total numbers of γ2b cells in the spleens of prolactin- and placebo-treated mice were 1.82 ± 0.76 × 10
6 and 1.67 ± 0.51 × 10
6, respectively. (
b) ELISpot assay showed that prolactin did not induce an increase in the number of anti-DNA antibody–secreting B cells (cells).
