Prolactin modulates the naive B cell repertoire
J. Clin. Invest. Elena Peeva, et al. 111:275 doi:10.1172/JCI16530 [
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Figure 3B cell maturation. (
a) Splenocytes from nontransgenic BALB/c mice treated with murine prolactin (
n = 5) or placebo (
n = 3) were stained for CD19, CD21, CD23, and HSA and were analyzed for T1, T2, marginal zone, and follicular subsets. B cell subsets were analyzed on the basis of data obtained with CD21 and HSA staining for the T1, T2, and follicular subsets and CD21 and CD23 staining for the marginal zone subset. (
b) In prolactin-treated mice, the numbers of immature HSA
high transitional B cells were reduced (
P = 0.002). In the mature HSA
low B cell population, the numbers of follicular (CD21
intermed/HSA
low) B cells were increased (
P = 0.002). (
c) The marginal zone B cell subset (CD21
high/CD23
low) was increased in prolactin-treated mice (
P = 0.005) (
d) ELISpot assay of follicular and marginal zone B cells pooled from four ovine prolactin–treated or four placebo-treated mice demonstrated an increase in the number of spontaneously secreting DNA-reactive follicular B cells. MZ, marginal zone; Fo, follicula.
