Prolactin modulates the naive B cell repertoire
J. Clin. Invest. Elena Peeva, et al. 111:275 doi:10.1172/JCI16530 [Go to this article.]

Figure 2
Analysis of peripheral B cell lymphocytes. Splenocytes were isolated from seven murine prolactin–treated and seven placebo-treated mice after 4 weeks of treatment. (a) A higher percentage of B cells express γ2b in prolactin-treated than in placebo-treated mice (P = 0.01). The total number of γ2b cells in the spleens of prolactin-treated mice was 3.98 ± 0.84 × 10⁶, as compared with 2.3 ± 0.53 × 10⁶ in placebo-treated mice. (b) γ2b staining of splenocytes Representative dot plot showing an increased number of γ2b-expressing cells in prolactin-treated mice. (c) Localization of IgG2b-expressing B cells. B cell regions in the spleen were identified with anti-IgM staining (blue); γ2b-expressing B cells were labeled with anti-γ2b (yellow). The spleens of prolactin-treated mice displayed an expansion of the γ2b B cells, which were found mainly in the follicles. In spleens of placebo-treated mice, transgene-bearing B cells were localized to the T/B cell interface.