Endothelium-derived Toll-like receptor-4 is the key molecule in LPS-induced neutrophil sequestration into lungs
J. Clin. Invest. Graciela Andonegui, et al. 111:1011 doi:10.1172/JCI16510 [Go to this article.]

Figure 6
L-selectin shedding from LPS-treated neutrophils differs between TLR4 chimeric mice. Whole blood drawn by cardiac puncture was untreated or treated with LPS for 30 minutes prior to analysis of L-selectin expression by neutrophils from EndotheliumTLR4^–/– mice (a) and LeukocyteTLR4^–/– mice (b). Flow cytometric analysis is shown for a nonspecific isotype control (broken line), L-selectin expression on untreated cells (thin solid line), and L-selectin expression on LPS-treated cells (heavy solid line). Results shown are representative of three experiments per mouse group using C57BL/6 vs. TLR4^–/– chimeric mice.