Endothelium-derived Toll-like receptor-4 is the key molecule in LPS-induced neutrophil sequestration into lungs
J. Clin. Invest. Graciela Andonegui, et al. 111:1011 doi:10.1172/JCI16510 [Go to this article.]

Figure 2
LPS-induced neutrophil sequestration into the lung capillaries of TLR4^+/+ mice. Mice were treated with LPS for 4 hours, and the lungs were prepared for histology or electron microscopy. (a) Leder (esterase) stain of lung sections. Untreated mice (left panel) show normal alveolar septa with capillaries (small arrows) and small venules (large arrow). No neutrophils are seen in this section. LPS-treated mice (right panel) show infiltrating neutrophils (arrowheads) within capillaries and venules. The inset shows several stained neutrophils in a venule. (b) Quantitative analysis of neutrophil sequestration into the different lung compartments. hpf, high-power field. (c) The electromicrograph shows several neutrophils (arrowheads) within capillaries. *P < 0.05 vs. untreated mice.