In vivo derivation of glucose-competent pancreatic endocrine cells from bone marrow without evidence of cell fusion
J. Clin. Invest. Andreea Ianus, et al. 111:843 doi:10.1172/JCI16502 [Go to this article.]

Figure 4
Cell sorting and fluorescence analysis. (a–d) Peripheral blood nucleated cells; (e–h) bone marrow cells; (i–l) isolated and dispersed islet cells. (a, e, and i) Representational scatter plot of side (x axis) and forward (y axis) scatter of respective cells. (b–d, f–h, and j–l) Fluorescence scans (x axis, EGFP; y axis, phycoerythrin [red] filter) of respective cells. (b, f, and j) Cells from INS*EGFP donor mice (experiment 1). (c, g, and k) Cells from irradiated wild-type mice transplanted with bone marrow from INS*EGFP mice (experiment 1). (d, h, and l) Cells from irradiated ROSA-stoplox-EGFP mice transplanted with bone marrow from INS2-CRE mice (experiment 2). No fluorescence is detectable in donor or recipient peripheral blood nucleated cells or bone marrow cells. EGFP is detectable in islets of donor animals of experiment 1 and in the recipients of experiment 1. In b–d, f–h, and j–l, cells in the line equidistant from both axes show some autofluorescence, and cells in the lower right quadrant have EGFP signal. See Figure 1 for description of experiments 1 and 2.