In vivo derivation of glucose-competent pancreatic endocrine cells from bone marrow without evidence of cell fusion
J. Clin. Invest. Andreea Ianus, et al. 111:843 doi:10.1172/JCI16502 [
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Figure 3Immunofluorescence and FISH of isolated, dispersed pancreatic islet cells from experiment 1 (see Figure
1). Images of identical fields are shown in the respective panels: (
a–
d) insulin, (
e–
h) IPF-1, and (
i–
l) HNF3β immunodetection. (
a,
e, and
i)Bright-field phase; (
b,
f, and
j) EGFP (note slight autofluorescence of control isolated islet cells); (
c,
g, and
k) Immunostaining with rhodamine X–labeled secondary antibody for panels. (
c) Insulin, (
g) IPF-1, (
k) HNF3β, and (
d h, and
l) FISH for Y chromosome (in yellow) and nucleus stain with DAPI (blue). Y chromosome is present only in EGFP-positive cells. Scale bar, 5 μm; ×630.
