In vivo derivation of glucose-competent pancreatic endocrine cells from bone marrow without evidence of cell fusion
J. Clin. Invest. Andreea Ianus, et al. 111:843 doi:10.1172/JCI16502 [Go to this article.]

Figure 2
Immunofluorescence and FISH for Y chromosome of pancreatic 5- to 7-μm frozen sections from recipient mice of experiments 1 and 2 (see Figure 1). (a and b) Bright-field phase images. (c and d) Composite overlay image of immunofluorescence for insulin (red) and EGFP (green), and FISH for Y chromosome (yellow) and DAPI staining of nuclei (blue). Y chromosome–positive cells (arrows) are present in the islet (i) and in the exocrine portions of the pancreas (e). EGFP is present only in insulin-positive cells in the islet. Scale bar, 10 μm; ×400. Insets in c and d are magnified areas of EGFP and insulin double-positive cells containing a Y chromosome.