The suppressor of cytokine signaling–1 (SOCS1) is a novel therapeutic target for enterovirus-induced cardiac injury
J. Clin. Invest. Hideo Yasukawa, et al. 111:469 doi:10.1172/JCI16491 [Go to this article.]

Figure 6
AAV-directed expression of dnSOCS1 inhibits the CVB3-mediated cytopathic effect. Hearts of mice were directly injected with AAV Myc-tagged dnSOCS1 or AAV LacZ. Two weeks later, the mice were infected with CVB3. Five days after infection, myocardial sections were stained with anti-Myc (a) or anti–β-galactosidase antibodies (d). Evans blue uptake was examined in the same section (b and e). The merged images (c and f) show that in the areas where there is expression of dnSOCS1, there is a lack of membrane disruption due to the virus infection, as compared with areas of the myocardium that express LacZ. The percent area of Evans blue dye staining in the regions of the myocardium that stained positive for dnSOCS1 or LacZ was quantitated from three mice per group (g) and was significantly decreased in dnSOCS1-expressing areas as compared with LacZ-expressing areas. Results are shown as means ± SE. *P < 0.01 for the comparison with LacZ-transduced areas. Scale bars: 1 mm (a–f).