Pacemaker channel dysfunction in a patient with sinus node disease
J. Clin. Invest. Eric Schulze-Bahr, et al. 111:1537 doi:10.1172/JCI16387 [Go to this article.]

Figure 3
Immunostaining of HCN4 and HCN4-573X in COS-7 cells. Wild-type and HCN4-573X channels were transiently expressed in COS-7 cells. For detection, wild-type HCN4 was HA-tagged and HCN4-573X was myc-tagged. Both channels showed comparable immunostains (a and b) with immunoreactivity detectable in perinuclear regions and at the plasma membrane. When coexpressed (c–f), the immunostaining patterns did not change and completely overlapped, and they were particularly concentrated in the cell membrane (f).