Sensitizing antigen-specific CD8⁺ T cells for accelerated suicide causes immune incompetence
J. Clin. Invest. Christoph Wasem, et al. 111:1191 doi:10.1172/JCI16344 [Go to this article.]

Figure 2
Effect of Bis VIII on antiapoptotic gene expression. Freshly isolated spleen cells (a) or day 5 T cell blasts (b) were stimulated with plate-bound anti-CD3 for 8 hours in the presence or absence of 5 μM Bis VIII. RNA was isolated and reverse transcribed, and gene expression was detected by PCR using serially diluted cDNA templates. (c) Detection of Fas, FasL, and cFLIP_L expression by real-time PCR. Day 5 T cell blasts were treated as indicated in (b). Fold induction of gene expression (mean values ± SD of triplicates) as compared with unstimulated controls are shown. *P < 0.001. (d) Detection of cFLIP_L protein by Western blot. T cell blasts were treated as described above. Equal protein loading was confirmed by anti–α-tubulin Western blot. Ratios between cFLIP_L and α-tubulin expression, normalized to the unstimulated control, are indicated.