Sensitizing antigen-specific CD8⁺ T cells for accelerated suicide causes immune incompetence
J. Clin. Invest. Christoph Wasem, et al. 111:1191 doi:10.1172/JCI16344 [Go to this article.]

Figure 1
CD4⁺ and CD8⁺ T cells are sensitized by Bis VIII for AICD. Highly purified CD4⁺ and CD8⁺ T cell blasts (day 5) were stimulated with plate-bound anti-CD3 in the presence or absence of increasing concentrations of Bis VIII. Apoptosis (AICD) was assessed by detection of fragmented apoptotic nuclei, as described in Methods. The Fas/FasL dependency of AICD was confirmed by adding neutralizing anti-FasL or anti–TNF-α antibodies. Mean values ± SD of triplicates are shown.