Endothelial lipase is a major determinant of HDL level
J. Clin. Invest. Tatsuro Ishida, et al. 111:347 doi:10.1172/JCI16306 [Go to this article.]

Figure 4
HDL lipoprotein particle analysis. (a) SDS-PAGE analysis of apoA-I in the HDL peak fractions isolated by FPLC. One hundred microliters of the HDL peak fraction from FPLC were evaluated by 15% SDS-PAGE. Gels were stained and quantitated by densitometry. (b) Western blot analysis of apoA-I in HDL peak fraction from FPLC. Images were quantitated by densitometry. (c) HDL isolated by density-gradient ultracentrifugation (d = 1.063–1.21) was evaluated by 12% SDS-PAGE, with 20 μg of protein loaded per lane. Lane 1 is human HDL standard, and lane 5 is a molecular weight marker. H, human HDL standard. (d) HDL isolated by density-gradient ultracentrifugation (d = 1.063–1.21) was evaluated by TLC. Equal amounts of HDL, as determined by protein quantitation, were loaded. KO, LIPG^–/–; Tg, hLIPGTg. *P <0.05 compared to WT.