Estrogen modulates cutaneous wound healing by downregulating macrophage migration inhibitory factor
J. Clin. Invest. Gillian S. Ashcroft, et al. 111:1309 doi:10.1172/JCI16288 [Go to this article.]

Figure 5
Treatment of MIF null wounds with TGF-β (200 ng per wound) results in delayed healing as compared with vehicle-treated wounds (a, top panels). Wounds are representative of day 3 after wounding. Arrows demarcate wound edges. Treatment of MIF null wounds with rhMIF results in impaired healing (a, bottom panels) (day 7 after wounding, 1-μg dose) as compared with vehicle (PBS). Scale bar represents 100 μm. Results represent means ± SEM (n = 5, *P < 0.05). (b) Neutralization of MIF in wild-type OVX mice with anti-MIF antibodies leads to improved healing as compared with vehicle alone (day 3 after wounding). Arrows demarcate wound edges, which cannot be visualized in the OVX mice. Graph illustrates wound areas at day 3 after wounding in a dose-response study with increasing doses of anti-MIF antibodies injected at the time of wounding. Scale bar represents 100 μm. Results represent means ± SEM (n = 5, *P < 0.05, **P < 0.01). C, no injection at wound site; PBS, vehicle control.