Vpr R77Q is associated with long-term nonprogressive HIV infection and impaired induction of apoptosis
J. Clin. Invest. Julian J. Lum, et al. 111:1547 doi:10.1172/JCI16233 [Go to this article.]

Figure 8
Apoptosis signaling in Jurkat cells infected with VSV-G pseudotype virus containing Vpr R77Q. Jurkat cells were mock infected (control) or infected with 0.01 MOI of VSV-G pseudotyped virus containing wild-type Vpr, Vpr R77Q, or VSV-G with Vpr negative (Vpr–). Cells were collected on days 2, 4, and 6 and analyzed for apoptosis by (a) light scatter (leftward shift) and (b) mitochondrial Δψ_m using DIOC₆. In parallel, cytosolic extracts from day-6 infections were immunoblotted for procaspase-8, procaspase-3, and DFF (c). Equal protein loading was confirmed by PCNA (data not shown).