Vpr R77Q is associated with long-term nonprogressive HIV infection and impaired induction of apoptosis
J. Clin. Invest. Julian J. Lum, et al. 111:1547 doi:10.1172/JCI16233 [Go to this article.]

Figure 5
Cytochrome c release from mitochondria treated with wild-type or R77Q Vpr. Jurkat T cells were treated with wild-type or R77Q (mutant) Vpr, lysed, and fractionated into mitochondrial and cytosolic fractions. The cytosolic fraction was blotted for cytochrome c (top), and each fraction was then blotted for the mitochondria-specific protein Hsp70 (bottom) to confirm no mitochondrial contamination within the cytosols.