JCI - Vpr R77Q is associated with long-term nonprogressive HIV infection and impaired induction of apoptosis

Vpr R77Q is associated with long-term nonprogressive HIV infection and impaired induction of apoptosis

Julian J. Lum, Oren J. Cohen, Zilin Nie, Joel G. Weaver, Timothy S. Gomez, Xiao-Jian Yao, David Lynch, André A. Pilon, Nanci Hawley, John E. Kim, Zhaoxia Chen, Michael Montpetit, Jaime Sanchez-Dardon, Eric A. Cohen, Andrew D. Badley

Published in Volume 111, Issue 10

J Clin Invest. 2003; 111(10):1547–1554 doi:10.1172/JCI16233

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Figure 1

R77Q Vpr induces less apoptosis in single-cycle infections using VSV-G pseudotype HIV-1 virus. (a) Jurkat T cells were infected at an MOI of 0.01 with VSV-G pseudotyped virus expressing wild-type Vpr, Vpr-negative (Vpr^–), or R77Q Vpr. Cells were analyzed by annexin V FITC/propidium iodide (PI) staining for apoptotic cells at the indicated time points after infection. Values are representative of three independent experiments. The inset denotes the number of propidium iodide–positive cells at each time point (% annextin⁺/PI^– refers to the percentage of cells which stain with annexin V-FITC, but not PI). (b) Cells were isolated on day 6 after infection and stained with TUNEL for apoptosis measurement and with p24 antibody for measurement of viral replication. Data are representative of two independent infections.