Jalaluddin Sheikh, John R. Czeczulin, Susan Harrington, Susan Hicks, Ian R. Henderson, Chantal Le Bouguénec, Pierre Gounon, Alan Phillips, James P. Nataro
RT-PCR for aap transcript in 042, 042aggR, and 042aggR(pBADaggR). Cultures were grown to late log phase in either L-broth with 0.5% glucose or L-broth with 0.5% arabinose. RT-PCR was performed as described in Methods, and products were separated on 1% agarose gels. The aap transcript migrates as a 0.3-kb species as predicted.