Suppressor of cytokine signaling-1 regulates acute inflammatory arthritis and T cell activation
J. Clin. Invest. Paul J. Egan, et al. 111:915 doi:10.1172/JCI16156 [Go to this article.]

Figure 7
Hyperresponsiveness of SOCS-1–deficient T cells in vitro. (a) Enhanced proliferation of draining LN cells from SOCS-1^–/– IFN-γ^–/– mice to mBSA. Popliteal and axillary LN cells were isolated from SOCS-1^+/+ IFN-γ^–/– and SOCS-1^–/– IFN-γ^–/– mice and cultured in vitro for 3 days in the presence or absence of mBSA. (b) Hyperresponsiveness of purified CD4⁺ T cells to anti-CD3. Splenic CD4⁺ T cells were purified from nonarthritic SOCS-1^+/+ IFN-γ^–/– mice (circles) and SOCS-1^–/– IFN-γ^–/– mice (squares) and cultured with increasing concentrations of immobilized anti-CD3 antibody. T cell proliferation was assessed after 3 days in culture by incorporation of ³H-TdR.