Suppressor of cytokine signaling-1 regulates acute inflammatory arthritis and T cell activation
J. Clin. Invest. Paul J. Egan, et al. 111:915 doi:10.1172/JCI16156 [
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Figure 5Phenotype of cells accumulating in the synovium of
SOCS-1–/– IFN-γ–/– mice during acute arthritis. Synovium was dissected from the knee joints of
SOCS-1+/+ IFN-γ–/– and
SOCS-1–/– IFN-γ–/– mice on day 7 after arthritis induction, and synovial cells were isolated by digestion with dispase and collagenase. (
a) Increased percentage of myeloid cells in the synovium of
SOCS-1–/– IFN-γ–/– mice during acute arthritis. Synovial cells were stained for expression of CD45, and analysis of CD11b and GR-1 expression was performed on CD45
+ cells. Results shown are representative of three independent experiments. (
b) Flow cytometric analysis of nonadherent synovial cells for expression of CD4 and CD8. Nonadherent synovial cells were isolated following overnight incubation. (
c) Cytocentrifuge preparations of joint exudate cells, isolated on day 7 following arthritis induction. ×400.
