Suppressor of cytokine signaling-1 regulates acute inflammatory arthritis and T cell activation
J. Clin. Invest. Paul J. Egan, et al. 111:915 doi:10.1172/JCI16156 [Go to this article.]

Figure 1
Immunohistochemical staining for SOCS-1 during acute inflammatory arthritis. Acute inflammatory arthritis was induced by intra-articular injection of 200 μg mBSA into the knee joint followed by three daily subcutaneous injections of 250 ng IL-1β. Mice were sacrificed on day 7 after mBSA injection. Paraffin-embedded joint sections were dewaxed and stained with purified goat polyclonal antibodies specific for SOCS-1 or goat polyclonal antibodies of irrelevant specificity. (a and b) Inflamed WT synovium, stained with antibodies to SOCS-1 (×100, a) or with an isotype control (×100, b). (c) Inflammatory synovial granuloma from a WT mouse, stained with antibodies to SOCS-1 (×400). (d and e) Synovial pannus from a WT mouse, stained with antibodies to SOCS-1 (×400, d) or with an isotype control (×400, e). Arrows in d indicate SOCS-1 positive synovial cells at the cartilage-pannus junction. (f) SOCS-1^–/– IFN-γ^–/– synovium stained with antibodies to SOCS-1 (×100). (g) Inflamed synovial granuloma from a SOCS-1^–/– IFN-γ^–/– mouse, stained with antibodies to SOCS-1 (×400).