Characterization of lymphocyte responses to peanuts in normal children, peanut-allergic children, and allergic children who acquired tolerance to peanuts
J. Clin. Invest. Victor Turcanu, et al. 111:1065 doi:10.1172/JCI16142 [Go to this article.]

Figure 7
Comparison of the responses of Ara h2–specific T lymphocytes from PA (n = 4) and NA donors (n = 4). PBMCs were isolated, CFSE labeled, and cultured in the presence of 20 μg/ml purified Ara h2 for 7 days, then the cytokine-producing phenotype of antigen-specific (CFSE^low) cells was assessed by flow cytometry. Th-subset skewing was determined as ratios of CFSE^low cell percentages that produce Th1-type (IFN-γ) or Th2-type (IL-4) cytokines (and, respectively, TNF-α and IL-13). Points represent data from different individuals, while bars show median values. Significance was determined using the Mann-Whitney test (***P < 0.001; **P < 0.02).