Characterization of lymphocyte responses to peanuts in normal children, peanut-allergic children, and allergic children who acquired tolerance to peanuts
J. Clin. Invest. Victor Turcanu, et al. 111:1065 doi:10.1172/JCI16142 [Go to this article.]

Figure 1
PBMC proliferation monitored using CFSE labeling. PBMCs from a PA donor were isolated, labeled with CFSE, and cultured in the presence of 100 μg/ml peanut extract (a-c), 200 μg/ml OVA (d-f), 50 μg/ml PPD (g-i), or without exogenous antigens (j-l). On days 3, 5, and 7 after setting the cultures, cells were collected, stained with anti-CD4 PE, and analyzed by FACS. Figures represent the percentage of (CFSE^low CD4⁺) cells included in the respective gates. Similar results were obtained in three other donors