Characterization of lymphocyte responses to peanuts in normal children, peanut-allergic children, and allergic children who acquired tolerance to peanuts
J. Clin. Invest. Victor Turcanu, et al. 111:1065 doi:10.1172/JCI16142 [
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Figure 1PBMC proliferation monitored using CFSE labeling. PBMCs from a PA donor were isolated, labeled with CFSE, and cultured in the presence of 100 μg/ml peanut extract (
a-
c), 200 μg/ml OVA (
d-
f), 50 μg/ml PPD (
g-
i), or without exogenous antigens (
j-
l). On days 3, 5, and 7 after setting the cultures, cells were collected, stained with anti-CD4 PE, and analyzed by FACS. Figures represent the percentage of (CFSE
low CD4
+) cells included in the respective gates. Similar results were obtained in three other donors