Inhibition of skin tumor growth and angiogenesis in vivo by activation of cannabinoid receptors
J. Clin. Invest. M. Llanos Casanova, et al. 111:43 doi:10.1172/JCI16116 [
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Figure 4Cannabinoid receptor activation induces skin tumor cell apoptosis. (
a) The tumorigenic epidermal cell lines PDV.C57 (open symbols) and HaCa4 (closed symbols) were cultured with 25 nM WIN-55,212-2 alone (circles), or in combination with 0.2 μM SR141716 (SR1) (squares), or 0.2 μM SR144528 (SR2) (triangles), and cell viability was determined (
n = 5). (
b) The nontransformed epidermal cell lines MCA3D (open circles) and HaCat (closed circles), as well as primary human keratinocytes (closed squares), were cultured with 25 nM WIN-55,212-2, and cell viability was determined (
n = 4). (
c and
d) PDV.C57 cells were cultured as described before, and oligonucleosomal DNA fragmentation (
c) (
n = 6), and TUNEL staining (
d) (one representative experiment of four) were assessed. *Significantly different (
P < 0.01) from control incubations.
